Pomacea Spreads to South Carolina

Editor’s Note – This essay was subsequently published as: Dillon, R.T., Jr. (2019d)  Pomacea spreads to South Carolina.  Pp 15 - 18 in The Freshwater Gastropods of North America Volume 4, essays on Ecology and Biogeography.  FWGNA Press, Charleston.

Word reached us last week that a population of Pomacea has become established in the vicinity of Myrtle Beach, SC, extending the range of this invasive pest north about 500 km. The South Carolina Department of Natural Resources is currently studying options for control.

At this time the introduction seems localized to a single pond in a trailer park in the town of Socastee, about 10 km W of Myrtle Beach. The area was quite poorly drained prior to development, and ditching and filling operations have resulted in the creation of several retention ponds in the neighborhood. The population of Pomacea was discovered by SCDNR personnel investigating complaints of excessive algal growth in these bodies of water.


On May 6 our colleague David Knott of the SCDNR reported "lots of P. insularum egg clutches (above) and three snails (two were copulating) in one of several ponds." Thanks to David for the photo below.

Most of us in this group are all too familiar with the damage to aquatic crops and macrophytic vegetation caused by populations of Pomacea introduced worldwide. The pest has spread throughout Florida since its initial introduction in the 1980s. In 2005 it appeared in South Georgia (Post of 11/05), where attempts to control it have not been notably successful.

Although we do not have any evidence regarding the origin of the South Carolina population, we speculate that the initial introduction may have come through the release of unwanted pets. Pomacea (of several species) were readily available in local aquarium stores until a few years ago, and hobbyists traveling up from Florida might easily transport wild-collected animals.

The pond inhabited by the South Carolina population drains through underground culverts toward the Intracoastal Waterway, a degraded habitat that would not suffer terribly from the release of molluscicides. But upstream just a few kilometers is the mouth of the Waccamaw River, still lovely in spots, leading north to Lake Waccamaw, the pristine home of several endemic species.

Come on, boys! Are we going to take back-sass from a bunch of fat snails in a scum pond (above)? This is South Carolina - let's nuke 'em!

The Classification of the Planorbidae

Editor's Note.  This essay was subsequently published as: Dillon, R.T., Jr. (2019b)  The classification of the Planorbidae.  Pp 127-135 in the Freshwater Gastropods of North America Volume 2, Essays on the Pulmonates.  FWGNA Press, Charleston.

The Planorbidae is the most diverse of the basommatophoran pulmonate families, including hundreds of species and dozens of higher taxa worldwide. Planorbids are the most successful freshwater pulmonates in the topics, the notorious Biomphalaria, Bulinus, and Indoplanorbis serving as the intermediate hosts of schistosomiasis in both the Old World and the New. It is a point of pride for us in North America, therefore, that the classification of this family generally recognized around the world today was heavily influenced by the work of a hometown boy, Frank Collins Baker of Urbana, Illinois (1).

Here we pick up a thread left dangling somewhat over a year ago, the life and career of F. C. Baker (1867-1942). Baker cultivated a comprehensive knowledge of freshwater pulmonate taxonomy, great skills as an anatomist, and a tremendous feel for the living organisms to which he devoted his life. And his (1945) "The Molluscan Family Planorbidae" was a masterwork (2).

Baker originally conceived of his monograph in two parts - a systematic review of the anatomy and classification of all higher planorbid taxa worldwide, recent and fossil, and a survey of the nominal species inhabiting the Americas. Although only a skeletal 16 pages of text for the second half of the project had been completed at the time of his death, together with a phenomenal 140 plates, the first half was sufficiently complete for his editor (H. J. van Cleave) to carry to publication posthumously.

Baker left us lovely anatomical illustrations and detailed morphological observations for 81 species and races of planorbids representing diverse taxa worldwide, together with ranges (both geological and geographical) and species lists. On this basis he proposed a classification of the family recognizing 4 subfamilies, 36 genera and 18 subgenera.

We have noted previously that Baker's taxonomy remained firmly rooted in 19th-century typology throughout his career. The quality of the science in his 1945 monograph of the Planorbidae was not substantially different from that in his 1911 treatment of the Lymnaeidae. But what made the 1945 work so special was its worldwide scope. Baker offered detailed anatomical observations for Pingiella and Polypylis from China, Intha and Indoplanorbis from India, and Planorbis, Anisus, Segmentina and Hippeutis from Europe, and included seven higher taxa known only as fossils. The work should be better known today than it is.

For our modern understanding of planorbid systematics has developed in close parallel to (but lagging slightly behind) our understanding of the Lymnaeids. And just as Baker's (1911) lymnaeid monograph was supplanted by Bengt Hubendick's (1951) masterpiece (3), so too was Baker's (1945) monograph of the Planorbidae supplanted by Hubendick in 1955 (4).


With his greater access to the African and Eurasian faunas and his skill with thin-section microscopy, the Riksmuseum's Bengt Hubendick was able to explore planorbid anatomy down to the finest detail. The classification he proposed ten years after Baker's was explicitly evolutionary (Above, Note 5), postulating ancestral and derived character states and hypothesizing phylogenetic relationships. But rather than setting aside all the earlier classifications and starting afresh, as he had done with the lymnaeids four years previously, Hubendick built directly upon the foundation that F. C. Baker had laid. He wrote, "It is not my intention to give a complete account of the morphology of the different planorbids. Baker (1945) has already presented a comprehensive monograph on the subject." So taking "the recent genera accepted by him as a starting point," Hubendick was able to re-monograph the entire family Planorbidae in just 90 pages. Hubendick's monograph of the Lymnaeidae, a much smaller family with but two genera, had required 223 (6).

Hubendick criticized Baker rather severely for neglecting two major planorbid groups, the African Bulinus and the South American Plesiophysa (7). And indeed Hubendick's classification began by recognized three subfamilies - the Planorbinae, the Bulininae, and the Plesiophysinae - the latter two of which Baker had omitted. But within the Planorbinae, Hubendick recognized 31 genera gathered into 6 - 9 "tribes," broadly agreeing with Baker's genera and subfamilies. Hubendick did not advocate subgenera.

Later in his life, Hubendick (1978) suggested that the ancylid limpets be might united with the planorbids into a gigantic "Ancyloplanorbidae," but this idea never caught on, at least in its proposed form (8). The concept has recently been revived by a number of molecular phylogenetic studies, which tend to confirm that the planorbids and the ancylids are both paraphyletic and interdigitated (9). The classifications implied by molecular data are jarringly different in some respects from those that have been established by common practice over the last 50 years (10), and time will be required to see what new system stabilizes.

Meanwhile, back in North America, Burch (11) adopted the Hubendick (1955) classification with a couple minor tweaks and one big shove. He subsumed the genus Armiger under Gyraulus following Meier-Brook (12), and substituted the older name Vorticifex for the younger synonym used by Hubendick, Parapholyx (13). In addition, Burch also advanced a rather dramatic change to the genus Helisoma that has baffled me and many of our colleagues for quite a few years.

Both Hubendick and Baker recognized the North American genus Helisoma as a large, natural group. Baker sorted roughly 77 species and subspecies of Helisoma (too many!) into four subgenera: Planorbella, Seminolina, Pierosoma, and Helisoma (ss). Burch trimmed the specific nomina down to about 17. Then without explanation or attribution, he raised Planorbella to the genus level and diverted 16 of the species (from three of Baker's former subgenera) into it. To the single species of Helisoma left behind (H. anceps) Burch added the single species of the Baker/Hubendick genus Carinifex, C. newberryi. No rationale was offered for any of the Helisoma rearrangements whatsoever.

During the course of my research for last month's essay, however, I stumbled across a clue to Burch's "mystery of the exploded Helisoma" - the 1966 monograph that Dwight Taylor published on the Plio/Pleistocene mollusk faunas of the American West (14). At the end of that lengthy work, in his section entitled "taxonomic notes," Taylor raised Baker's Planorbella to the genus level and removed the Helisoma exactly as Burch was to advocate ten years later, offering as his rationale the apparent axis of shell coiling (15). Baker's 530 pages of anatomical observations seem to have been dismissed by Taylor, and he was apparently unaware of Hubendick's monograph entirely (16). Variance on a single shell character was sufficient for D. W. Taylor to explode the genus Helisoma, and apparently Burch found Taylor's evidence convincing.

The FWGNA project has adopted a (slightly updated) version of the Hubendick (1955) system for the classification of the (roughly 45) species of planorbids inhabiting North America, preserving Helisoma and Carinifex as recognized by Baker. We do not mean to imply that a 50-year-old hypothesis is (or could be!) the definitive model of planorbid evolution. Indeed, we think it quite likely that future malacologists may adopt some classification that combines the ancylids and planorbids along the lines that Hubendick himself foresaw in 1978. But for now, this is it:

[The Classification of the Planorbidae]

Notes

(1) The Legacy of Frank Collins Baker  [20Nov06]

(2) Baker, F. C. (1945) The Molluscan Family Planorbidae. University of Illinois Press, Urbana. 530 pp.

(3) The Classification of the Lymnaeidae [28Dec06]

(4) Hubendick, B. (1955) Phylogeny in the Planorbidae. Trans. Zool. Soc. London 28: 453-542

(5) Hubendick's "synoptic diagram" is depicted above [click here for an enlargement]. Although one certainly sees evolutionary trees proposed for fossil taxa in papers of this era, I do think that Hubendick's construction of phylogenetic trees for entirely modern taxa was much ahead of his time.

(6) But in fairness, the Hubendick (1951) lymnaeid monograph went down to the species level. Neither Baker nor Hubendick seems to have contemplated reviewing the immense worldwide diversity of planorbids any lower than the genus.

(7) And to be fair to Baker, I think he probably considered "Bulinidae" and "Pleisiophysidae" separate families.

(8) Hubendick, B. (1978) Systematics and comparative morphology of the Basommatophora. pp 1 - 47 in "Pulmonates, Volume 2A," (V. Fretter & J. Peake, eds). Academic Press, New York.

(9) Morgan, J.A.T. et al. (2002) A phylogeny of planorbid snails, with implications for the evolution of Schistosoma parasites. Mol. Phylogenet. Evol. 25: 477-488. Jorgensen, A., T. K. Kristensen & J. R. Stothard (2004) An investigation of the "Ancyloplanorbidae" (Gastropoda, Pulmonata, Hygrophila): preliminary evidence from DNA sequence data. Molec. Phylogenet. Evol. 32: 778-787. Albrecht, C., K. Kuhn & B. Streit (2007) A molecular phylogeny of Planorboidea (Gastropoda, Pulmonata): Insights from enhanced taxon sampling. Zoologica Scripta 36: 27 - 39.

(10) Albrecht and colleagues propose that Bulinus and Indoplanorbis be split out into a separate Bulinidae, and that the most of the ancylid limpets might best be considered a subfamily within an enlarged Planorbidae.

(11) Burch originally proposed his classification for the North American freshwater gastropods in 1978 (Journal de Conchyliologie 115: 1-9). His "North American Freshwater Snails" was published as an EPA manual in 1982, as three volumes of Walkerana (1980, 1982, 1988), and as a stand-alone book in 1989.

(12) Meier-Brook, C. (1979) The planorbid genus Gyraulus in Eurasia. Malacologia 18: 67 - 72. Meier-Brook, C. (1983) Taxonomic studies on Gyraulus (Gastropoda: Planorbidae). Malacologia 24: 1 - 113.

(13) Parapholyx (Hanna 1922) was apparently a bit more prominent in Hubendick's day, but Vorticifex (Meek 1870) clearly has priority. See Burch's Note #60.

(14) Taylor, D. W. (1966) Summary of North American Blancan nonmarine mollusks. Malacologia 4: 1 - 172.

(15) While understanding that the group he called "Planorbella" bore sinistral shells, Taylor considered the shells of Carinifex and Helisoma anceps to be dextral. In point of fact, all planorbids are embryonically sinistral, but the shells of some species flip over their backs ("hyperstrophically") so as to appear more or less dextral in adults.

(16) Actually, I suspect this was an intentional snub. See the illuminating anecdote about Hubendick in the D. W. Taylor obituary, Malacologia 50: 175-218.

Red Flags, Water Resources, and Physa natricina

Editor’s Note – This essay was subsequently published as: Dillon, R.T., Jr. (2019d) Red Flags, Water Resources, and Physa natricina.  Pp 153 - 158 in The Freshwater Gastropods of North America Volume 4, Essays on Ecology and Biogeography.  FWGNA Press, Charleston.

This past December brought the publication of a brief paper by our colleagues Christopher Rogers and Amy Wethington synonymizing the federally listed “Snake River Physa” (Physa natricina) under the cosmopolitan P. acuta (1). How a local population of an invasive pest came to be protected under the Endangered Species Act is but one blunder in the sad history of fumbles and missteps that has characterized the record of American Malacology in the Snake River Canyon of southern Idaho. Can anything be learned to prevent such embarrassments in the future?

The misadventure began in the early 1980s, when Idaho Power Company proposed the construction of six new hydroelectric projects on the middle Snake River, perhaps to impound the last free-flowing reaches of a 122 mile section already tightly controlled by 11 dams. Environmental groups rose up in opposition (2), and I would freely confess sympathy for their cause. I have a visceral love of rivers and the lotic biota, and hate impoundments because they are ugly, stinking blights, all too rapidly infested with Bud-swilling bass fishermen.

But insults to the public aesthetic will never be as compelling to the permitting agencies as hydropower, irrigation, and jobs, no matter how egregious the choice of beer. Thus it is not a coincidence that within ten years of the announcements by Idaho Power, five species of endangered freshwater gastropods were discovered in the middle Snake River. Pyrgulopsis idahoensis, Valvata utahensis, Taylorconcha serpenticola, Physa natricina, and the undescribed "Banbury Springs lanx" were added to the federal list of endangered and threatened wildlife on December 14, 1992 (3). The Idaho Power hydro projects were shelved.

About "Pyrgulopsis idahoensis" we have written much in recent years (4). Although originally believed endemic to the Snake River, it proved to be a junior synonym of P. robusta, its actual range extending over four states. Far from being endangered, the Snake River population of P. robusta may be the largest single population of freshwater gastropods on earth. Taylorconcha and V. utahensis are also not rare, and are currently being studied for delisting as well (5). And now published is the paper by Rogers & Wethington sinking Physa natricina.

Physa natricina was described in 1988 by Dwight Taylor, a reclusive millionaire whose 44-page obituary will appear in the next Malacologia (6). Although perhaps better qualified as a paleontologist, Taylor often published on the modern terrestrial and freshwater malacofauna of the American West. He is best remembered for his fanciful treatments of the Physidae (7) and the Cuatro Cienegas hydrobiids (8), imagining more higher taxa than valid biological species actually exist to sort into them.

To be fair, Taylor’s 1988 work (9) conformed to the same 19th century standards of practice under which most elements of America's molluscan fauna have been described. He did distinguish his Physa natricina from P. gyrina, a strikingly different animal which is very common in the Snake River. But the brief comparison he offered between his new species and P. integra, the synonym for P. acuta most commonly applied in the upper Midwest, should have raised a red flag. Taylor wrote that the penial sac of P. integra "is more slender, with a kink near its distal end, and is not bent near the middle." Kinks and bends in mollusk anatomy? Was Taylor nuts? (10) One need not be a malacologist - one need only to have eaten an oyster - to realize that anybody who would distinguish the internal anatomy of a gastropod by reference to kinks and bends is simply unqualified for his profession.

Taylor's work was generally characterized by false precision. For example, in his introductory description of the (entire!) subgenus Physa, he stated that the "spawn capsule...is up to about 10 mm long with 20 eggs." But even under controlled conditions here in my laboratory, we commonly record individual Physa egg masses ranging from over 100 embryos to fewer than 1. Meanwhile, about truly important matters Taylor seems to have been careless at best. His statement that the natricina holotype was deposited in the Los Angeles County Museum ("LACM 2256") seems to have been a fabrication. Christopher Rogers was finally able to track down Taylor's P. natricina holotype at the California Academy of Sciences, where it was not deposited until 1999.

And here's another red flag - the hypothesized rarity of the new species. Taylor's original description was based on but two live-collected animals, "despite arduous effort" to obtain more. But species do not exist as individuals - they exist in populations. Any generally-trained biologist might well wonder how a population as sparse as P. natricina seems to have been for over 20 years could remain viable.
.
In late 2005 I was pleased to accept an invitation from the Bureau of Reclamation to visit the Minidoka Dam (photo above) on the Snake River east of Rupert, Idaho, for a Physa strategy meeting. Also present were our colleagues Amy Wethington, John Keebaugh, Steve Lysne, and several others. The product of that meeting was a consensus that a sequencing project should be undertaken as soon as the next fresh P. natricina individual might be recovered from the river – a day which never arrived (11). But on the basis of what I was able to learn about that elaborately managed river system, together with my own limited observations of the environment and my general experience with the biology of physids, I offer the following hypothesis (12).

I suggest that the two individual P. acuta from which Taylor described his “Physa natricina” in 1988 may have been flushed into the main Snake River from irrigation ditches. In addition to power generation, many of the Snake River dams serve to divert irrigation water to the surrounding farm land. This is done seasonally, and both the peak diversions and the peak release flows back to the river can be high. I suggest that irrigation waters may sporadically carry elements of the canal-dwelling macrobenthos into the Snake River, including occasional individual Physa acuta.

Science is a self-correcting process. It is gratifying to see two of our own, Rogers and Wethington, designing the research program and publishing the paper that has turned us back from our 20-year blunder. But at such a cost! Literally millions of dollars have been wasted monitoring, managing, and protecting a snail that anyone on six continents could find in the ditch behind his local McDonalds, licking special sauce off the hamburger wrappers. Can we avoid even the first step down such paths in the future?

Yes, if we watch for red flags. And the biggest red flag waving over the Physa natricina blunder was not the vacuous description, the false precision, or the biological implausibility of the phantom snail's very existence. The biggest red flag was that this entire research program was motivated, from its very inception, by water resource politics.

You have heard me preach this sermon before - science and politics do not mix. When the two worldviews collide, compromises must be made, and it's always the science that suffers, in my experience. Malacology was corrupted at least four times by water resource politics in the middle Snake River 20 years ago. And science continues to be corrupted in our professional organizations, from the AAAS to the NAS, on matters ranging from global climate to stem cells. But when we see that red flag fly, we must stop.

Notes

(1) Rogers, D. C. & A. R. Wethington (2007) Physa natricina Taylor 1988, junior synonym of Physa acuta Draparnaud, 1805 (Pulmonata: Physidae). Zootaxa 1662: 45-51. A pdf reprint can be requested from the author.

(2) Wuerthner, G. (1992) No Home for Snails. Defenders May/June 92: 8 - 14.

(3) US Fish & Wildlife Service (1992). Endangered and threatened wildlife and plants; Determination of endangered or threatened status for five aquatic snails in south central Idaho. 50 CFR Part 17. Federal Register 57(240)59244-57. (December 14, 1992)

(4) I've offered four previous posts on the Snake River Pyrgulopsis: Idaho Springsnail Showdown [28Apr05], Idaho Springsnail Panel Report [23Dec05], When Pigs Fly in Idaho [30Jan06], and FWS finding on the Idaho Springsnail [4Oct06].

(5) More Snake River Gastropods Studied for Delisting [14June07]

(6) Kabat, A. R. & R. I. Johnson (2008) Dwight Willard Taylor (1932-2006): His life and malacological research. Malacologia 50: 175-218.

(7) Wethington, A. R. & C. Lydeard (2007) A molecular phylogeny of Physidae (Gastropoda: Basommatophora) based on mitochondrial DNA sequences. J. Moll. Stud. 73: 241-257.

(8) Hershler, R. (1985) Systematic revision of the Hydrobiidae (Gastropoda: Rissoacea) of the Cuatro Cienegas Basin, Coahuila, Mexico. Malacologia 26: 31 - 123.

(9) Taylor, D. W. (1988) New species of Physa (Gastropoda: Hygrophila) from the western United States. Malac. Rev. 21: 43-79.

(10) Yes.

(11) See the Bureau of Reclamation's web site for the "Physa Amendment" to its "2004 Biological Assessment and Opinions for Operations and Maintenance of Reclamation Projects in the Snake River Basin above Brownlee Reservoir." There's also an (8/05) "Implementation Plan for Proposed Snake River Physa Surveys" available toward the bottom of the page.

(12) I don’t remember who first advanced this hypothesis – it was very likely in existence long before my introduction to the matter. And I’m pretty sure I’m not the only one who holds it, but I wouldn't presume to speak for anybody else.

Semisulcospira II: A Second Message from The East

Last month we reviewed the experiments of Urabe (1998, 2000) vividly demonstrating that shell morphology in the East Asian pleurocerid genus Semisulcospira has a substantial non-genetic component. Since the taxonomy of this important group is based almost entirely on its shell, such results suggest that the true number of biological species in the genus Semisulcospira may be much different from the number of nomina conventionally applied.

This month we’ll update that message with a second chapter from Taehwan Lee, Dairmaid O’Foighil, and two colleagues from Pai Chai University in Daejon, South Korea (1). What might the application of modern molecular techniques add to our understanding of evolution in this enigmatic genus from The East?

The range of Semisulcospira libertina extends from Japan onto the Korean Peninsula, where it shares rivers and streams with ten other nominal Semisulcospira species, as judged by their shell morphology. Lee and his colleagues sampled eight of those species from nine sites – libertina (four populations), coreana (two populations), extensa (two populations), and single populations of forticosta, gottschei, multicincta, nodiperda, and tegulata. They sequenced two genes, the mitochondrial 16S and the nuclear 28S ribosomal genes, in most cases just one individual per population but in some cases up to ten or even twenty per population. They built phylogenetic trees using three different methods (maximum parsimony, maximum likelihood, and Baysian) to mollify all constituencies among their reviewers (2), with a couple other genera of Korean pleurocerids added as outgroups (3).

It should come as no surprise, to all of us familiar with Urabe’s work, that the phylogenetic trees obtained by Taehwan, Dairmaid and their colleagues showed a mish-mash of nominal species bearing almost no relationship to the traditional taxonomy based on shell. Only Semisulcospira extensa appeared monophyletic. Populations of the other seven species displayed a genetic structure we have seen much of recently (4) – heterogeneous mixtures with numerically predominant modal clades and the scattered highly-divergent genotypes that (in my post of 7/07) I have called "sports."

Not only were the nominal species polyphyletic, the populations and even the individual snails were polyphyletic. I am ashamed to confess that I was entertained by the author’s discovery of a single S. libertina individual heterozygous at the 28S locus. I should have been compassionate – analysis of this individual snail was apparently a real pain in the can. Taehwan and colleagues had to clone the PCR products amplified from this individual in plasmid vectors and sequence a bunch of recombinant bacterial colonies. The two alleles turned out to differ by 23 nucleotides, leaving the single individual S. libertina that bore them splayed across opposite ends of their 28S maximum likelihood tree. I think I may read phylogenetic systematic research for the same reason I watch NASCAR - the wrecks (5).

Taehwan, Dairmaid and their colleagues reviewed the same list of explanations for this phenomenon that they previously examined in their paper on Laevapex with Andrea Walther in the lead (6) – paralogous mt markers, cryptic species, introgression, and (their choice) retention of ancestral polymorphism through incomplete lineage sorting (7). They concluded with the recommendation that coreana, forticosta, gottschei, multicincta, nodiperda, and tegulata all be synonymized under the single, phenotypically variable biological species Semisulcospira libertina, broadly distributed across East Asia.

In summary, the messages borne to us this month by Taehwan, Dairmaid and their colleagues from The East are that “biologists interested in freshwater cerithiodean molecular phylogenetics approach these taxa as potential morphospecies complexes,” and that in the future “meaningful phylogenetic study of these organisms may well require the use of both mitochondrial and nuclear markers together with population level samples of all nominal taxa within regional drainages.” Message received, guys! And in other news … Ronald Reagan elected President (8).

Notes

1) Lee, T., H. C. Hong, J. J. Kim and D. O’Foighil (2007) Phylogenetic incongruence involving nuclear and mitochondrial markers in Korean populations of the freshwater snail genus Semisulcospira (Cerithioidea: Pleuroceridae). Molec. Phylog. Evol. 43: 386-397.

2) The tiny fringe group who prefers neighbor-joining techniques is not likely to read MP&E, much less referee their manuscripts.

3) It will be recalled from last month’s essay that Semisulcospira is ovoviviparous. As outgroups, Lee et al. selected four species from two strictly oviparous genera of Korean pleurocerids - Hua and Koreoleptoxis.

4) The parallel between these findings and our own (2004) results is striking, although perhaps not of the same magnitude. Dairmaid tells me that the sequence divergence ranged up to 8.9% within his Semisulcospira populations, while we reported up to 14.5% intrapopulation 16S sequence divergence in American Goniobasis. See Dillon & Frankis, Amer. Malac. Bull 19:69-77.

5) With Joe Gibbs running Toyotas in 2008, it may not be too much longer until Sprint Cup fans receive our own “message from The East.”

6) Walther, A., T. Lee, J. B. Burch, and D. Ó Foighil. 2006. E Pluribus Unum: A phylogenetic and phylogeographic reassessment of Laevapex (Pulmonata: Ancylidae), a North American genus of freshwater limpets. Molecular Phylogenetics and Evolution, 40: 501-516.

7) That’s close, but I don’t think exactly right. The likelihood that an ancestral polymorphism will be preserved in a pair of sorted lineages decreases with internodal time, which may be great in populations as old as these. I think such polymorphisms may not the product of incomplete lineage sorting, but rather have evolved endogenously in large, ancient, and isolated populations. And I think independently-evolved genes may converge on the same adaptive peak, or may be collected outside the population cluster in which they evolved by long branch attraction.

8) Dillon, R. T. and G. M. Davis (1980) The Goniobasis of southern Virginia and northwestern North Carolina: Genetic and shell morphometric relationships. Malacologia 20: 83-98.

Semisulcospira Research: A Message from The East

'Tis the season for Messages from The East. And in recent years I have taken some inspiration from research conducted on Semisulcospira, a genus of pleurocerid snails widespread throughout Japan, Korea, Taiwan and eastern China. As a potential intermediate host for the lung fluke Paragonimus westermani, Semisulcospira has become one of the better-studied of all the freshwater prosobranchs.

In many of the details of its general biology, life history, and habitat, Semisulcospira reminds me a great deal of our North American Goniobasis or Elimia. Scores of species have been described (30 from Japan alone, according to Davis, note 1), almost entirely on the basis of shell morphology. The most striking difference between Goniobasis and Semisulcospira is that the latter is a brooder - holding eggs in a modified pallial oviduct until hatch. But although ovoviviparity is often associated with parthenogenesis in freshwater gastropods, I'm not aware of any evidence of asexual reproduction in Semisulcospira (2).

Ovoviviparous reproduction does, however, facilitate laboratory rearing studies. And in 1998 and 2000, Misako Urabe (of Nara Womens' University) published a pair of papers describing a set of experiments directly exploring the heritability of shell morphology in Semisulcospira that deserve to be more widely known.

Urabe worked with Semisulcospira reiniana, an extremely variable species co-occurring with (and sometimes indistinguishable from) the more widely distributed S. libertina in the Kamo River of Kyoto. The plate at left (from Davis, see Note 1) shows both S. libertina (figs 1-4) and S. reiniana (figs 5 & 6) from several Japanese populations (scale bar in mm).

In the 1998 work, Urabe (3) used mother/offspring regression to estimate the heritability of the three shell shape parameters of Raup (4): S (the roundness of the generating curve), W (the rate of whorl expansion), and T (the rate of whorl translation). In the wild, snails collected from more rapid currents tended to have larger W and smaller T, that is, larger body whorls and lower spires (5). But lab experiments showed the heritabilities of all three shell shape parameters to be nonsignificant - no different from zero.

In the 2000 work, Urabe (6) reared juveniles to 6.8 mm under standard conditions, marked sibships with waterproof tags, and then divided them into two aquaria - one with a sand substrate and the other with pebbles. After one year, snails were scored by their "rib intensity," a 0/1 coding system for shell sculpture developed by the author. Urabe showed both that mothers with stronger shell ribbing tended to produce higher percentages of ribbed offspring, but that snails grown to maturity in the tank with the sand substrate tended to form ribbed shells more frequently than those grown on pebbles.

Quite a few researchers have directly demonstrated shell phenotypic plasticity using pulmonate snails as models. Amy Krist published a nice experiment showing that crayfish predation can affect aperture shape in Goniobasis livescens a couple years ago (7). But the experiments of Urabe are the best demonstrations of phenotypic plasticity in freshwater prosobranchs of which I am aware.

So to what extent might we expect Urabe's conclusions for Semisulcospira to generalize to the pleurocerids of North America? The evidence is all around us that they do. I've seen populations of Goniobasis catenaria inhabiting Piedmont streams in North Carolina, for example, that intergrade seamlessly from strongly carinate shells to shells almost entirely smooth, apparently in correlation with the substrate (8). And I dedicated my entire post in February 2007 to a phenomenon I called "Goodrichian Taxon Shift," the broadening and thickening of shell morphology demonstrated by populations of North American pleurocerids as rivers widen, deepen, and slow. Which brings us to our "Message from The East."

In recent years, modern research programs have led to substantial taxonomic revisions in the Asian Pleuroceridae. At least 17 of the 30 specific nomina of Semisulcospira described from Japan on the basis of shell character had been synonymized under S. libertina by 1972, according to Davis (9). The clear implication of Urabe's work, that such trends will continue, has recently been borne out, as we shall see. And might the long-benighted pleurocerid fauna of North America also be overdue for a taxonomic revision along similar lines? Again, said the Zen Master, we will see.

Notes

(1) Davis, G. M. 1969. A taxonomic study of some species of Semisulcospira in Japan (Mesogastropoda: Pleuroceridae). Malacologia 7: 211-294.

(2) Males seem to occur in high frequency in all populations.

(3) Urabe, M. 1998. Contribution of genetic and environmental factors to shell shape variation in the lotic snail Semisulcospira reiniana (Prosobranchia: Pleuroceridae) J. Moll. Stud. 64: 329-343.

(4) Raup, D. M. 1966. Geometric analysis of shell coiling: general problems. J. Paleontol. 40: 1178-1190.

(5) To be quite precise - snails active in fast currents at night tended to show the shell shape relationship best. Apparently Semisulcospira populations are nocturnal, much like the Melanoides in my goldfish bowl. Urabe suggested that, since shell growth only occurs when the mantle edge is flush with the aperture, "it is only the environment that a snail experiences when it is active at night-time that affects shell formation." Interesting!

(6) Urabe, M. 2000. Phenotypic modulation by the substratum of shell sculpture in Semisulcospira reiniana (Prrosobranchia: Pleuroceridae). J. Moll. Stud. 66: 53-59.

(7) Krist, A. C. 2002. Crayfish induce a defensive shell shape in a freshwater snail. Invert. Biol. 121: 235-242.

(8) The smooth form is given the subspecific name "dislocata," while the strongly carinate form is G. catenaria s.s.

(9) Davis, G. M. 1972. Geographic variation in Semisulcospira libertina (Mesogastropoda: Pleuroceridae). Proc. Malac. Soc. Lond. 40: 5-32.

Symposium at NABS '08

To the FWGNA group,

Our good friend Bill Clark is co-organizing symposium on sampling low-density populations for the annual meeting of the North American Benthological Society next year in Salt Lake City, May 25 - 30. See Bill's message below. His [PDF] flier is available from the FWGNA site.

Bill and his colleague Leska Fore have been working on the endangered freshwater gastropods of the Snake River. They're hoping to share new methods, discuss unique sampling issues related to benthic species, and perhaps even break into a spontaneous discussion of the future of the ESA. Bill invited any of us who might wish to contribute a paper to this symposium to get in touch with Leska at the contact information below. Looks like a great opportunity!

We'll keep in touch,
Rob


-------------

Subject: Symposium announcement
Date: Mon, 19 Nov 2007 13:32:16 -0500
From: "Clark, William" WilliamClark@idahopower.com
To: "Dillon Jr, Robert T."

Hi Rob:
I wonder if you could send this information for a symposium announcementout to your FWGNA group mailing list? I've attached a one page flyer and the basic information is also presented below in the email.

Thank you very much,
Bill Clark (and Leska Fore)

William H. Clark,
Macroinvertebrate Biologist
Idaho Power Company
P.O. Box 70
Boise, Idaho 83707 USA
tel: 208-388-2689
FAX: 208-388-6902
email:WilliamClark@idahopower.com

______________________________

Quantitative Methods for Evaluating the Status of Threatened Species
Organizers: Leska S. Fore, Statistical Design, Inc. & William H. Clark,Idaho Power Co.
Contact: Leska Foreleska@seanet.com
206 632-4635

Many of the benthic freshwater species identified to be at risk for extinction, e.g., mussels, clams, and snails, may be rare, unevenly distributed, or hard to detect. The focus of this session is on the quantitative methods used to assess population size, condition, orchange, such as mark-recapture, multi-stage survey sampling, and adaptive sampling. The goal of this special session is to bring together practitioners working with at risk populations to compare the limitations and advantages of various methods for different geographic settings and different types of organisms. Results from these studies can have enormous economic impact; for example, when power generation is limited at hydroelectric facilities to protect a threatened species. This session is not limited to any particular species group or any particular method of population assessment. General methods papers related to sampling are also welcome. Of greatest interest are studies in which the scientific results are embedded in the decision process forspecies conservation and protection.--

Leska S. Fore
Statistical Design
136 NW 40th St.
Seattle, WA 98107
(206) 632-4635 phone
(206) 632-3752 fax
www.seanet.com/~leska

Ducks, Snails, and Worms - When Invasive Species Conspire!

Editor’s Note – This essay was subsequently published as: Dillon, R.T., Jr. (2019d)  Ducks, snails, and worms - When invasive species conspire!  Pp 11 - 14 in The Freshwater Gastropods of North America Volume 4, essays on Ecology and Biogeography.  FWGNA Press, Charleston.

Freshwater snails have suffered a spate of bad press in the upper Midwest recently. Late last week our friend Henry Fieldseth sent us an article from the Minneapolis Star Tribune (6Nov07, pasted below) attributing the death of thousands of waterfowl in a local lake to infections by trematode worms, with the "banded mystery snail" (Viviparus georgianus) indicted as a co-conspirator. This is not the first mass murder rap to be pinned on a digenean trematode or an invasive gastropod henchman, nor will it be the last, I fear.

Massive late-summer mortalities of waterfowl attributable to trematodiasis have been reported at least since the 1960s, when thousands of ducks were apparently killed by mixed infections of the digeneans Cyathocotyle bushiensis and Sphaeridiotrema globulus in the St. Lawrence River south of Quebec. Hoeve & Scott (1) linked these deaths to ingestion by the ducks of the invasive European gastropod Bithynia tentaculata. In 1997, tens of thousands of coots were killed in Wisconsin's Shawano Lake by a third digenean worm, the European Leyogonimus polyoon, again carried by Bithynia (2). And since 2002, all three worms have been implicated in massive waterfowl mortalities around Lake Onalaska, a backwater of the upper Mississippi River near La Crosse (3). All three of these digenean trematodes, the introduced Leyogonimus as well as the natives.
.
Cyathocotyle and Sphaeridiotrema, display Type III life cycles – entering their ultimate (vertebrate) host by ingestion of an infected meal (4). The vertebrate host sheds eggs through its feces, which hatch into ciliated miricidia and penetrate the first intermediate host, a freshwater gastropod. (The photo of Bithynia tentaculata at left was taken by Lars Peters.) The miricidium develops numerous sack-like rediae in its first intermediate host, each of which gives rise to many cercariae. Then the cercariae swim out of the snail to find a second intermediate host, which may be selected from among a wide variety of aquatic animals – a second snail or an aquatic insect, for example. (Typically substantial specificity is demonstrated for the first intermediate host, but not for the second.) The definitive vertebrate host (a duck or coot in this case) becomes infected with mature worms when it ingests the second intermediate host.

Trematodes do not typically kill their definitive hosts. The massive mortalities we have seen scattered through diverse waterfowl populations in the upper Midwest are most likely attributable to unusually high rates of snail ingestion as well as to double or exotic infections. So since the ducks (with their digenean parasites) disperse readily on continent-level scales, federal and state agencies have signaled their intent to focus their efforts to control the trematodiasis by controlling the snails (5).

Thus last week's discovery of the massive waterfowl mortality attributable to Cyathocotyle and Sphaeridiotrema in Minnesota's Lake Winnibigoshish is especially bad news. For if the initial reports hold up, here the first intermediate host of the worm is not Bithynia, but rather Viviparus georgianus (6). Viviparus georgianus is a native of the American southeast, and is much more widely distributed throughout the United States than Bithynia. And if the problem trematodes can infect Viviparus, they would also seem likely to be able to exploit Bellamya (Cipangopaludina) and Campeloma, and in so doing expand their potential ranges nationwide.

I am often asked about the potentially negative effects of artificially-introduced freshwater gastropod populations. My usual response has been that such effects are difficult to establish. Very few studies have ever demonstrated community or ecosystem effects attributable to exotic freshwater gastropods – they seem to invade environments that are already disturbed and compete rarely (if ever) with native populations.

But here splashed across our morning newspapers we read that "carcasses dropped by eagles hung in the trees on the island, and feathers littered the shore" (LaCrosse Tribune 28Oct07, link removed). This is a dramatic example of unfortunate environmental consequences directly attributable to the spread of exotic freshwater gastropods. And at this point I don't think that snail control will solve the problem. And I really wish I could think of any other alternative.

We'll keep in touch,
Rob


Notes

(1) Hoeve, J. and M. E. Scott (1988) Ecological studies on Cyathocotyle bushiensis (Digenea) and Sphaeridiotrema globulus (Digenea), possible pathogens of dabbling ducks in southern Quebec. J. Wild. Diseases 24: 407 - 421.

(2) Exotic parasite causes large scale mortality in American coots. USGS-NWHC Fact Sheet 6/2001. [PDF]

(3) Exotic parasite of American coot discovered in exotic snail in Lake Onalaska. USGS-NWHC Bulletin 07-01. [PDF]

(4) See my Chapter 6 of Dillon (2000) for a more complete review of trematode life cycles, from the standpoint of the gastropod. Although most of that chapter (perhaps unsurprisingly) focuses on Type I Fasciola and Type II Schistosoma, there is a bit about Type III Echinostoma as well.

(5) Finding the exotic faucet snail (Bithynia tentaculata): Investigation of waterbird die-offs on the upper Mississippi River National Wildlife and Fish Refuge. USGS-NWHC report 2007-1065. [PDF]

(6) Scaup and Coot die-off at Lake Winnibigoshish. Minnesota DNR:
http://www.dnr.state.mn.us/hunting/waterfowl/scaup.html



------[Minneapolis Star Tribune 6Nov07]---------

Nov. 6: Parasite has killed thousands of scaup
The deadly organism has infected snails at Lake Winnibigoshish and has been picked up by the ducks, who dive below water to feed. The DNR is concerned the parasite might spread to other lakes.
By Doug Smith, Star Tribune
Last update: November 08, 2007 – 4:49 PM

A parasite has killed thousands of ducks on Lake Winnibigoshish in northern Minnesota, and could kill many more before the fall migration is over."We picked up 1,000 dead scaup [also known as bluebills] on Saturday," said Steve Cordts, Minnesota Department of Natural Resources waterfowl specialist.

He saw many other scaup still alive but unable to fly, or to fly far. "You could boat right up to them," Cordts said. Perhaps 3,000 ducks, mostly scaup, and some coots have died in the past week. "I'm sure there's more dead birds," Cordts said Tuesday.The ducks apparently are dying from trematodes, a tiny 1-millimeter intestinal parasite or fluke that has infected snails in the lake. Scaup -- a duck that dives below water to feed -- eat the snails, then are infected. "They essentially bleed to death," Cordts said.

The parasite was confirmed in scaup and coots sent to the National Wildlife Health Center in Madison, Wis. Similar die-offs caused by trematodes have occurred spring and fall since 2002 on the Mississippi River near La Crosse, Wis., killing about 40,000 ducks and coots since then. Die-offs again are occurring there this fall.

Cordts said he's not sure how the trematodes made it to Winnie. They apparently have infected a snail called the banded mystery snail, which was first found on Winnie about eight years ago. They are infecting the faucet snail on the Mississippi.

Officials aren't sure how many ducks might eventually die on Winnie, or what impact, if any, it will have on the scaup population. But Cordts is concerned that the snails and parasites might spread to other Minnesota waters. Other duck species also could eat the snails and become infected, he said.

There is concern because the continental scaup population has been declining since 1984 and hit an all-time low last year at about 3 million. Hunters annually kill about 300,000. Minnesota hunters killed about 20,000 last year. Lake Winnibigoshish is a major scaup resting area during migration. "We could have 20,000 scaup show up on Winnie right now," Cordts said. "If that happens, they'd pretty much all be at risk."

Cordts plans to check the lake again today, but he won't collect any more dead ducks. Instead, carcasses will be left to decompose or be eaten by scavengers. The parasite apparently is not a threat to other species, including humans, but Cordts said hunters shouldn't eat sick waterfowl.

Doug Smith • dsmith@startribune.com
© 2007 Star Tribune. All rights reserved.